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humanomniexpress-24 microarray chip  (Illumina Inc)


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    Structured Review

    Illumina Inc humanomniexpress-24 microarray chip
    Humanomniexpress 24 Microarray Chip, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+microarray/humanomniexpress+24++microarray+chip/us12367981-351-19-23
    Average 90 stars, based on 1 article reviews
    humanomniexpress-24 microarray chip - by Bioz Stars, 2026-11
    90/100 stars

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    Generated:

    Article Title: Conservation of Native Livestock Breeds in Russia: Current State and Promising Prospects.
    Article Snippet: .. Application of three bioinformatics approaches (computation of FST values, detection of ROH islands and hapFLK method) to analyze whole-genome SNP genotyping data generated using the high-density (HD) DNA microarray Bovine HD BeadChip (Illumina) aided in identifying genomic regions under selection pressure in two oldest native cattle breeds, Kholmogory and Yaroslavl. ..

    Article Title: Conservation of Native Livestock Breeds in Russia: Current State and Promising Prospects
    Article Snippet: .. Application of three bioinformatics approaches (computation of F ST values, detection of ROH islands and hapFLK method) to analyze whole-genome SNP genotyping data generated using the high-density (HD) DNA microarray Bovine HD BeadChip (Illumina) aided in identifying genomic regions under selection pressure in two oldest native cattle breeds, Kholmogory and Yaroslavl. ..

    Microarray:

    Article Title: Conservation of Native Livestock Breeds in Russia: Current State and Promising Prospects.
    Article Snippet: .. Application of three bioinformatics approaches (computation of FST values, detection of ROH islands and hapFLK method) to analyze whole-genome SNP genotyping data generated using the high-density (HD) DNA microarray Bovine HD BeadChip (Illumina) aided in identifying genomic regions under selection pressure in two oldest native cattle breeds, Kholmogory and Yaroslavl. ..

    Article Title: Conservation of Native Livestock Breeds in Russia: Current State and Promising Prospects
    Article Snippet: .. Application of three bioinformatics approaches (computation of F ST values, detection of ROH islands and hapFLK method) to analyze whole-genome SNP genotyping data generated using the high-density (HD) DNA microarray Bovine HD BeadChip (Illumina) aided in identifying genomic regions under selection pressure in two oldest native cattle breeds, Kholmogory and Yaroslavl. ..

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Article Title: Classifier apparatus with decision support tool
    Article Snippet: .. Genotypes for the DNA SNP locations were determined from enrolled subjects' saliva specimens by testing using the Illumina HumanOmniExpress-24® microarray chip and associated Illumina assay reagents and instrumentation (measurement device 141). ..

    Article Title: Robust molecular subgrouping and reference-free aneuploidy detection in medulloblastoma using low-depth whole genome bisulfite sequencing.
    Article Snippet: .. This has happened three times since MNP was launched, as Illumina replaced the 450k microarray chip with EPIC, EPIC + and now EPICv2.0 versions. ..

    Selection:

    Article Title: Conservation of Native Livestock Breeds in Russia: Current State and Promising Prospects.
    Article Snippet: .. Application of three bioinformatics approaches (computation of FST values, detection of ROH islands and hapFLK method) to analyze whole-genome SNP genotyping data generated using the high-density (HD) DNA microarray Bovine HD BeadChip (Illumina) aided in identifying genomic regions under selection pressure in two oldest native cattle breeds, Kholmogory and Yaroslavl. ..

    Article Title: Conservation of Native Livestock Breeds in Russia: Current State and Promising Prospects
    Article Snippet: .. Application of three bioinformatics approaches (computation of F ST values, detection of ROH islands and hapFLK method) to analyze whole-genome SNP genotyping data generated using the high-density (HD) DNA microarray Bovine HD BeadChip (Illumina) aided in identifying genomic regions under selection pressure in two oldest native cattle breeds, Kholmogory and Yaroslavl. ..

    Biomarker Discovery:

    Article Title: A Comparative Study of Medium-coverage Genome Sequencing and SNP Array Technology in Identifying Chromosomal Abnormalities to Advance Prenatal and Postnatal Diagnosis.
    Article Snippet: .. Hayes JL, Tzika A, Thygesen H, Berri S, Wood HM, Hewitt S, Pendlebury M, Coates A, 379 Willoughby L, Watson CM, Rabbitts P, Roberts P, Taylor GR: Diagnosis of copy number 380 variation by Illumina next generation sequencing is comparable in performance to 381 oligonucleotide array comparative genomic hybridisation. .. Hayes JL, Tzika A, Thygesen H, Berri S, Wood HM, Hewitt S, Pendlebury M, Coates A, 379 Willoughby L, Watson CM, Rabbitts P, Roberts P, Taylor GR: Diagnosis of copy number 380 variation by Illumina next generation sequencing is comparable in performance to 381 oligonucleotide array comparative genomic hybridisation.

    Next-Generation Sequencing:

    Article Title: A Comparative Study of Medium-coverage Genome Sequencing and SNP Array Technology in Identifying Chromosomal Abnormalities to Advance Prenatal and Postnatal Diagnosis.
    Article Snippet: .. Hayes JL, Tzika A, Thygesen H, Berri S, Wood HM, Hewitt S, Pendlebury M, Coates A, 379 Willoughby L, Watson CM, Rabbitts P, Roberts P, Taylor GR: Diagnosis of copy number 380 variation by Illumina next generation sequencing is comparable in performance to 381 oligonucleotide array comparative genomic hybridisation. .. Hayes JL, Tzika A, Thygesen H, Berri S, Wood HM, Hewitt S, Pendlebury M, Coates A, 379 Willoughby L, Watson CM, Rabbitts P, Roberts P, Taylor GR: Diagnosis of copy number 380 variation by Illumina next generation sequencing is comparable in performance to 381 oligonucleotide array comparative genomic hybridisation.

    Hybridization:

    Article Title: A Comparative Study of Medium-coverage Genome Sequencing and SNP Array Technology in Identifying Chromosomal Abnormalities to Advance Prenatal and Postnatal Diagnosis.
    Article Snippet: .. Hayes JL, Tzika A, Thygesen H, Berri S, Wood HM, Hewitt S, Pendlebury M, Coates A, 379 Willoughby L, Watson CM, Rabbitts P, Roberts P, Taylor GR: Diagnosis of copy number 380 variation by Illumina next generation sequencing is comparable in performance to 381 oligonucleotide array comparative genomic hybridisation. .. Hayes JL, Tzika A, Thygesen H, Berri S, Wood HM, Hewitt S, Pendlebury M, Coates A, 379 Willoughby L, Watson CM, Rabbitts P, Roberts P, Taylor GR: Diagnosis of copy number 380 variation by Illumina next generation sequencing is comparable in performance to 381 oligonucleotide array comparative genomic hybridisation.

    Transfection:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Negative Control:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Immunoprecipitation:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Polymerase Chain Reaction:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Purification:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Sensitive Assay:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    RNA Library Preparation:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Chromatin Immunoprecipitation:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Article Title:
    Article Snippet: .. The ChIP DNA Library was sequenced with Illumina HiSeq X Ten using the paired-end module and with 150 bp reads on each end (Novogene Biotech, China). ..

    RNA Sequencing:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Gene Expression:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    Derivative Assay:

    Article Title: Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human PPARγ mouse mAb Wakabayashi et al. 23 IgG-A3409 Anti-mouse JMJD1A mouse mAb Abe et al. 8 ; Takahashi et al. 9 ; Abe et al. 10 IgG-F0231, IgG-F0618 Anti-NFIC rabbit pAb Proteintech Cat#16399-1-AP; RRID: AB_2878252 Anti-F4/80 rabbit pAb Proteintech Cat#28463-1-AP; RRID: AB_2881149 Anti-H3K27ac mouse mAb Kimura et al. 49 9E2H19 Anti-H3K9me2 mouse mAb Kimura et al. 49 6D11 Anti-H3K9me3 mouse mAb Kimura et al. 49 2F3 Anti-H3K27me3 rabbit pAb Merck Millipore Cat#07–449; RRID: AB_310624 Anti-V5 mouse mAb Thermo Fisher Scientific Cat#R960-25; RRID: AB_2556564 Anti-Histone H3 Abcam Cat#ab1791; RRID: AB_302613 Anti-IDH1 goat pAb Santa Cruz Cat#sc-49996; RRID: AB_2123166 Anti-IDH2 mouse mAb Abcam Cat#ab55271; RRID: AB_943793 Anti-IDH3α rabbit pAb Abcam Cat#ab58641; RRID: AB_2295799 Anti-IDH3β goat pAb Santa Cruz Cat#sc-55674; RRID: AB_2123291 Anti-IDH3γ goat pAb Novus Biologicals Cat#NBP1-78786; RRID: AB_11029416 Anti-mouse IgG-HRP Sigma-Aldrich Cat#A4416; RRID: AB_258167 Anti-rabbit IgG-HRP Sigma-Aldrich Cat#A0545; RRID: AB_257896 Anti-goat IgG-HRP Sigma-Aldrich Cat#A5420; RRID: AB_258242 Bacterial and virus strains Escherichia Coli DH5α Toyobo DNA-903 Chemicals, peptides, and recombinant proteins DMEM High Glucose Sigma-Aldrich Cat#D6429 DMEM without glucose, L-glutamine Sigma-Aldrich Cat#D5030 DMEM/Ham’s F12 medium Sigma-Aldrich Cat#D0547 Fetal Bovine Serum Thermo Fisher Scientific Cat#10270 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#09367-34 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat#I5879 Dexamethasone Sigma-Aldrich Cat#D4902 Insulin Sigma-Aldrich Cat#I5523 Indomethacin Sigma-Aldrich Cat#I7378 T3 Sigma-Aldrich Cat#T2877 Rosiglitazone Wako Cat#184-02651 Puromycin Sigma-Aldrich Cat#P8833 Oil Red O Nacalai Tesque Cat#25633-92 DL-Isocitric acid trisodium salt hydrate Sigma-Aldrich Cat#I1252 β-Nicotinamide adenine dinucleotide hydrate Sigma-Aldrich Cat#N7004 β-Nicotinamide adenine dinucleotide phosphate hydrate Sigma-Aldrich Cat#N5755 Dimethyl-α-ketoglutarate Sigma-Aldrich Cat#349631 Triethanolamine Sigma-Aldrich Cat#90279 Manganese(II) sulfate monohydrate Sigma-Aldrich Cat#M7634 (Continued on next page) Cell Reports 44, 116060, August 26, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat#34075 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Stealth RNAi siRNA Negative Control Med GC Duplex #2 Thermo Fisher Scientific Cat#12935-112 KOD Plus Neo DNA polymerase Toyobo Cat#KOD-401 ISOGEN Reagent Nippon Gene Cat#315-02504 Formaldehyde Solution FUJIFILM Wako Cat#064-00406 Ethylene glycol bis(succinimidylsuccinate) Thermo Fisher Scientific Cat#21565 Dynabeads Protein G for Immunoprecipitation Thermo Fisher Scientific Cat#10004D Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Critical commercial assays QIAquick PCR Purification Kit Qiagen Cat#28106 Qubit Double-stranded DNA High Sensitivity Assay Kit Thermo Fisher Scientific Cat#32854 TruSeq RNA Library Preparation Kit v2 Illumina Cat#RS-122-2001 or RS-122-2002 TruSeq ChIP Library Preparation Kit Illumina Cat#IP-202-1012 KAPA Hyper Prep Kit Kapa Biosystems Cat#KK8502 Deposited data RAW and analyzed RNA-seq data This paper GEO: GSE266318 RAW and analyzed ChIP-seq data This paper GEO: GSE266318 Gene expression microarray data This paper GEO: GSE266318 ChIP-seq data in 3T3-L1 Matsumura et al. 33 GEO: GSE73434 RNA-seq in scWAT Ito et al. 35 GEO: GSE236733 ChIP-seq data of ChREBP Jang et al. 37 GEO: GSE160605 Metabolome data in Figure 1D Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003976 Metabolome data in Figure 2B Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003977 Metabolome data in Figure 2I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003978 Metabolome data Figure 2K Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003979 Metabolome data in Figure S1H Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003985 Metabolome data in Figure S1I Metabolomics Workbench, NIH Project ID: PR002491 (Project https://doi.org/10.21228/M8NN8C); Study ID: ST003987 Experimental models: Cell lines 3T3-L1 ATCC ATCC CL-173 Plat-E Cosmo Bio RV-101 im-scWAT cells derived from Jmjd1a +/+ mice Ito et al. 35 N/A im-scWAT cells derived from Jmjd1a HY/HY mice Ito et al. 35 N/A Experimental models: Organisms/strains Mouse: Jmjd1a HY/HY Ito et al. 35 N/A Mouse: Jmjd1a flox/flox Kuroki et al. 50 N/A Mouse: Pdgfra-Cre Jackson Laboratories #013148 (Continued on next page) 18 Cell Reports 44, 116060, August 26, 2025 .. Mice were maintained on a chow diet (CE-2, CLEA Japan, Tokyo, Japan) with free access to water under a 12-h light/dark cycle at a constant temperature (22 ◦ C–24 ◦ C).

    DNA Array:

    Article Title: Genome-Wide Association Studies and Candidate Genes for Egg Production Traits in Layers from an F 2 Crossbred Population Produced Using Two Divergently Selected Chicken Breeds, Russian White and Cornish White.
    Article Snippet: The NanoDrop-2000 device (Thermo Fisher Scientific) was used to estimate the OD260/280 ratio in order to verify the isolated DNA’s purity. .. WGG of hens was conducted using the Illumina Chicken iSelect BeadChip DNA array containing ~60K SNPs. ..



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